E-test also known as epsilometer test is an exponential gradient testing methodology. The E-test which is quantitative method for an antibiotic sensitivity testing applies both dilution of antibiotic and diffusion of antibiotic into the media. E-test strip has concentration gradient of antibiotic with lowest concentration at lower region and highest concentration at topmost region. Following incubation, elliptical or symmetrical inhibition zone is formed. The intersection of the inhibitory zone edge and calibrated strip indicates the MIC value. It is simple, reliable and accurate method to determine MIC for wide spectrum of infectious agents.
Determining MIC by E test
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